Abstract

We present a new tomographic phase microscopy (TPM) approach that allows capturing the three-dimensional refractive index structure of single cells in suspension without labeling, using 180° rotation of the cells. This is obtained by integrating an external off-axis interferometer for wide-field wave front acquisition with holographic optical tweezers (HOTs) for trapping and micro-rotation of the suspended cells. In contrast to existing TPM approaches for cell imaging, our approach does not require anchoring the sample to a rotating stage, nor is it limited in angular range as is the illumination rotation approach. Thus, it allows noninvasive TPM of suspended live cells in a wide angular range. The proposed technique is experimentally demonstrated by capturing the three-dimensional refractive index map of yeast cells, while collecting interferometric projections at an angular range of 180° with 5° steps. The interferometric projections are processed by both the filtered back-projection method and the diffraction theory method. The experimental system is integrated with a spinning disk confocal fluorescent microscope for validation of the label-free TPM results.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Video-rate processing in tomographic phase microscopy of biological cells using CUDA

Gili Dardikman, Mor Habaza, Laura Waller, and Natan T. Shaked
Opt. Express 24(11) 11839-11854 (2016)

Optically driven full-angle sample rotation for tomographic imaging in digital holographic microscopy

Yu-chih Lin, Hui-Chi Chen, Han-Yen Tu, Chin-Yu Liu, and Chau-Jern Cheng
Opt. Lett. 42(7) 1321-1324 (2017)

Multiplexed force measurements on live cells with holographic optical tweezers

Cecile O. Mejean, Andrew W. Schaefer, Eleanor A. Millman, Paul Forscher, and Eric R. Dufresne
Opt. Express 17(8) 6209-6217 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (4)

» Media 1: AVI (4338 KB)     
» Media 2: AVI (4326 KB)     
» Media 3: AVI (2214 KB)     
» Media 4: AVI (2081 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription