Abstract

Holographic microscopy is an emerging biological technique that provides amplitude and quantitative phase imaging, though the contrast provided by many cell types and organelles is low, and until now no dyes were known that increased contrast. Here we show that the metallocorrole Ga(tpfc)(SO3H)2, which has a strong Soret band absorption, increases contrast in both amplitude and phase and facilitates tracking of Escherichia coli with minimal toxicity. The change in phase contrast may be calculated from the dye-absorbance spectrum using the Kramers–Kronig relations, and represents a general principle that may be applied to any dye or cell type. This enables the use of holographic microscopy for all applications in which specific labeling is desired.

© 2015 Optical Society of America

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Supplementary Material (5)

NameDescription
» Visualization 1: AVI (5281 KB)      Control cells
» Visualization 2: AVI (4825 KB)      Cells 0.2 uM dye
» Visualization 3: AVI (5442 KB)      Cells 0 uM dye
» Visualization 4: AVI (91 KB)      Control tumble
» Visualization 5: AVI (166 KB)      Dye tumble

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Figures (4)

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Equations (3)

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