Abstract

Temporal focusing (TF) microscopy is a wide-field two-photon excitation fluorescence (2PEF) microscopy technique, the optical sectioning capability of which is lower than that of point-scanning 2PEF microscopy. Here we demonstrate TF microscopy using three-photon excitation fluorescence (3PEF), which enhances the optical sectioning capability. As an excitation light source for the 3PEF, we developed an Yb-fiber chirped pulse amplifier, which produces 92-fs 9.0-μJ 1060-nm pulses at a repetition rate of 200 kHz. The optical sectioning capability was improved by a factor of 1.3 compared with that of 2PEF-TF microscopy. We also demonstrate dual-color imaging with both 2PEF and 3PEF.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Interferometric temporal focusing microscopy using three-photon excitation fluorescence

Keisuke Toda, Keisuke Isobe, Kana Namiki, Hiroyuki Kawano, Atsushi Miyawaki, and Katsumi Midorikawa
Biomed. Opt. Express 9(4) 1510-1519 (2018)

Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination

Keisuke Isobe, Takanori Takeda, Kyohei Mochizuki, Qiyuan Song, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Akiko Kumagai, Atsushi Miyawaki, and Katsumi Midorikawa
Biomed. Opt. Express 4(11) 2396-2410 (2013)

Two-photon microscopy using an Yb3+-doped fiber laser with variable pulse widths

Dong Uk Kim, Hoseong Song, Woosub Song, Hyuk-Sang Kwon, Miae Sung, and Dug Young Kim
Opt. Express 20(11) 12341-12349 (2012)

References

  • View by:
  • |
  • |
  • |

  1. D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
    [Crossref] [PubMed]
  2. G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005).
    [Crossref] [PubMed]
  3. O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express 2(3), 696–704 (2011).
    [Crossref] [PubMed]
  4. L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
    [Crossref] [PubMed]
  5. K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
    [Crossref] [PubMed]
  6. H. Choi, E. Y. S. Yew, B. Hallacoglu, S. Fantini, C. J. R. Sheppard, and P. T. C. So, “Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination,” Biomed. Opt. Express 4(7), 995–1005 (2013).
    [Crossref] [PubMed]
  7. E. Tal, D. Oron, and Y. Silberberg, “Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing,” Opt. Lett. 30(13), 1686–1688 (2005).
    [Crossref] [PubMed]
  8. A. Egner and S. W. Hell, “Time multiplexing and parallelization in multifocal multiphoton microscopy,” J. Opt. Soc. Am. A 17(7), 1192–1201 (2000).
    [Crossref] [PubMed]
  9. D. Fittinghoff, P. Wiseman, and J. Squier, “Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy,” Opt. Express 7(8), 273–279 (2000).
    [Crossref] [PubMed]
  10. A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
    [Crossref] [PubMed]
  11. Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
    [Crossref] [PubMed]
  12. N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
    [Crossref] [PubMed]
  13. D. Oron and Y. Silberberg, “Harmonic generation with temporally focused ultrashort pulses,” J. Opt. Soc. Am. B 22(12), 2660–2663 (2005).
    [Crossref]
  14. L.-C. Cheng, N. G. Horton, K. Wang, S.-J. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
    [Crossref] [PubMed]
  15. G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
    [Crossref] [PubMed]
  16. J. Widengren, U. Mets, and R. Rigler, “Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study,” J. Phys. Chem. 99(36), 13368–13379 (1995).
    [Crossref]
  17. G. Olivié, D. Giguère, F. Vidal, T. Ozaki, J.-C. Kieffer, O. Nada, and I. Brunette, “Wavelength dependence of femtosecond laser ablation threshold of corneal stroma,” Opt. Express 16(6), 4121–4129 (2008).
    [Crossref] [PubMed]
  18. J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
    [Crossref] [PubMed]
  19. H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
    [Crossref]
  20. Z. Zhao and Y. Kobayashi, “Ytterbium fiber-based, 270 fs, 100 W chirped pulse amplification laser system with 1 MHz repetition rate,” Appl. Phys. Express 9(1), 012701 (2016).
    [Crossref]
  21. L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
    [Crossref]
  22. Y. Chiba, H. Takada, K. Torizuka, and K. Misawa, “65-fs Yb-doped fiber laser system with gain-narrowing compensation,” Opt. Express 23(5), 6809–6814 (2015).
    [Crossref] [PubMed]
  23. J. Zhao, W. Li, C. Wang, Y. Liu, and H. Zeng, “Pre-chirping management of a self-similar Yb-fiber amplifier towards 80 W average power with sub-40 fs pulse generation,” Opt. Express 22(26), 32214–32219 (2014).
    [Crossref] [PubMed]
  24. K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
    [Crossref]
  25. X. Zhou, D. Yoshitomi, Y. Kobayashi, and K. Torizuka, “Generation of 28-fs pulses from a mode-locked ytterbium fiber oscillator,” Opt. Express 16(10), 7055–7059 (2008).
    [Crossref] [PubMed]
  26. T. Tanabe, F. Kannari, F. Korte, J. Koch, and B. Chichkov, “Influence of spatiotemporal coupling induced by an ultrashort laser pulse shaper on a focused beam profile,” Appl. Opt. 44(6), 1092–1098 (2005).
    [Crossref] [PubMed]
  27. E. Desurvire, “Analysis of gain difference between forward- and backward-pumped erbium-doped fiber amplifiers in the saturation regime,” IEEE Photonics Technol. Lett. 4(7), 711–714 (1992).
    [Crossref]
  28. A. Nagler, “Plossl type eyepiece for use in astronomical instruments,” US Patent 4,482,217 (1984).
  29. M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing for axial scanning,” Opt. Express 14(25), 12243–12254 (2006).
    [Crossref] [PubMed]
  30. J. Kapuściński and K. Yanagi, “Selective staining by 4’, 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels,” Nucleic Acids Res. 6(11), 3535–3542 (1979).
    [Crossref] [PubMed]
  31. K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
    [Crossref]

2017 (1)

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

2016 (1)

Z. Zhao and Y. Kobayashi, “Ytterbium fiber-based, 270 fs, 100 W chirped pulse amplification laser system with 1 MHz repetition rate,” Appl. Phys. Express 9(1), 012701 (2016).
[Crossref]

2015 (2)

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

Y. Chiba, H. Takada, K. Torizuka, and K. Misawa, “65-fs Yb-doped fiber laser system with gain-narrowing compensation,” Opt. Express 23(5), 6809–6814 (2015).
[Crossref] [PubMed]

2014 (2)

2013 (3)

2012 (1)

2011 (1)

2010 (2)

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

2008 (2)

2007 (2)

L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
[Crossref]

G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[Crossref] [PubMed]

2006 (1)

2005 (5)

2001 (1)

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

2000 (2)

1995 (2)

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

J. Widengren, U. Mets, and R. Rigler, “Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study,” J. Phys. Chem. 99(36), 13368–13379 (1995).
[Crossref]

1992 (1)

E. Desurvire, “Analysis of gain difference between forward- and backward-pumped erbium-doped fiber amplifiers in the saturation regime,” IEEE Photonics Technol. Lett. 4(7), 711–714 (1992).
[Crossref]

1979 (1)

J. Kapuściński and K. Yanagi, “Selective staining by 4’, 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels,” Nucleic Acids Res. 6(11), 3535–3542 (1979).
[Crossref] [PubMed]

Aubé, B.

Barber, P. R.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

Brunette, I.

Buhr, H. J.

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Carman, R. J.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

Chang, C.-Y.

Chang, N.-S.

Chen, S.-J.

Cheng, L.-C.

Chiba, Y.

Chichkov, B.

Cho, K.-C.

Choi, H.

Clark, C. G.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

Côté, D.

Dawes, J. M.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

Desurvire, E.

E. Desurvire, “Analysis of gain difference between forward- and backward-pumped erbium-doped fiber amplifiers in the saturation regime,” IEEE Photonics Technol. Lett. 4(7), 711–714 (1992).
[Crossref]

Dong, C. Y.

Donnert, G.

G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[Crossref] [PubMed]

Durst, M.

Durst, M. E.

Eggeling, C.

G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[Crossref] [PubMed]

Egner, A.

Fantini, S.

Fittinghoff, D.

Germer, C.-T.

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Giguère, D.

Hallacoglu, B.

Hanna, D. C.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

Hashimoto, H.

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Hell, S. W.

G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[Crossref] [PubMed]

A. Egner and S. W. Hell, “Time multiplexing and parallelization in multifocal multiphoton microscopy,” J. Opt. Soc. Am. A 17(7), 1192–1201 (2000).
[Crossref] [PubMed]

Hirosawa, K.

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

Horton, N. G.

L.-C. Cheng, N. G. Horton, K. Wang, S.-J. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
[Crossref] [PubMed]

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

Isbert, C.

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Isobe, K.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Kane, S.

L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
[Crossref]

Kannari, F.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

T. Tanabe, F. Kannari, F. Korte, J. Koch, and B. Chichkov, “Influence of spatiotemporal coupling induced by an ultrashort laser pulse shaper on a focused beam profile,” Appl. Opt. 44(6), 1092–1098 (2005).
[Crossref] [PubMed]

Kapuscinski, J.

J. Kapuściński and K. Yanagi, “Selective staining by 4’, 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels,” Nucleic Acids Res. 6(11), 3535–3542 (1979).
[Crossref] [PubMed]

Kawano, H.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Kieffer, J.-C.

Kobat, D.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

Kobayashi, Y.

Z. Zhao and Y. Kobayashi, “Ytterbium fiber-based, 270 fs, 100 W chirped pulse amplification laser system with 1 MHz repetition rate,” Appl. Phys. Express 9(1), 012701 (2016).
[Crossref]

X. Zhou, D. Yoshitomi, Y. Kobayashi, and K. Torizuka, “Generation of 28-fs pulses from a mode-locked ytterbium fiber oscillator,” Opt. Express 16(10), 7055–7059 (2008).
[Crossref] [PubMed]

Koch, J.

Koninck, P. D.

Korte, F.

Kumagai, A.

Kuznetsova, L.

L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
[Crossref]

Li, W.

Lin, C.-Y.

Liu, Y.

Mackechnie, C. J.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

Mets, U.

J. Widengren, U. Mets, and R. Rigler, “Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study,” J. Phys. Chem. 99(36), 13368–13379 (1995).
[Crossref]

Midorikawa, K.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Misawa, K.

Miyawaki, A.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Mizuno, H.

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Mochizuki, K.

Müller, G.

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Nada, O.

Nakamura, A.

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

Olivié, G.

Oron, D.

Ozaki, T.

Pagès, S.

Pask, H. M.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

Rigler, R.

J. Widengren, U. Mets, and R. Rigler, “Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study,” J. Phys. Chem. 99(36), 13368–13379 (1995).
[Crossref]

Ritz, J.-P.

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Roggan, A.

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Schaffer, C. B.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

Shank, C. V.

Sheppard, C. J. R.

Silberberg, Y.

So, P. T. C.

Song, Q.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

Squier, J.

L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
[Crossref]

D. Fittinghoff, P. Wiseman, and J. Squier, “Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy,” Opt. Express 7(8), 273–279 (2000).
[Crossref] [PubMed]

Suda, A.

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Takada, H.

Takeda, T.

Tal, E.

Tanabe, T.

Tanaka, M.

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

Therrien, O. D.

Toda, K.

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

Torizuka, K.

Tropper, A. C.

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

van Howe, J.

Vaziri, A.

Vidal, F.

Wang, C.

Wang, K.

L.-C. Cheng, N. G. Horton, K. Wang, S.-J. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
[Crossref] [PubMed]

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

Widengren, J.

J. Widengren, U. Mets, and R. Rigler, “Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study,” J. Phys. Chem. 99(36), 13368–13379 (1995).
[Crossref]

Wise, F. W.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
[Crossref]

Wiseman, P.

Xu, C.

Yanagi, K.

J. Kapuściński and K. Yanagi, “Selective staining by 4’, 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels,” Nucleic Acids Res. 6(11), 3535–3542 (1979).
[Crossref] [PubMed]

Yen, W.-C.

Yew, E. Y. S.

Yoshitomi, D.

Zeng, H.

Zhao, J.

Zhao, Z.

Z. Zhao and Y. Kobayashi, “Ytterbium fiber-based, 270 fs, 100 W chirped pulse amplification laser system with 1 MHz repetition rate,” Appl. Phys. Express 9(1), 012701 (2016).
[Crossref]

Zhou, X.

Zhu, G.

Zipfel, W.

Appl. Opt. (1)

Appl. Phys. B (1)

L. Kuznetsova, F. W. Wise, S. Kane, and J. Squier, “Chirped-pulse amplification near the gain-narrowing limit of Yb-doped fiber using a reflection grism compressor,” Appl. Phys. B 88(4), 515–518 (2007).
[Crossref]

Appl. Phys. Express (1)

Z. Zhao and Y. Kobayashi, “Ytterbium fiber-based, 270 fs, 100 W chirped pulse amplification laser system with 1 MHz repetition rate,” Appl. Phys. Express 9(1), 012701 (2016).
[Crossref]

Biomed. Opt. Express (4)

IEEE J. Sel. Top. Quantum Electron. (2)

H. M. Pask, R. J. Carman, D. C. Hanna, A. C. Tropper, C. J. Mackechnie, P. R. Barber, and J. M. Dawes, “Ytterbium-doped silica fiber lasers: versatile sources for the 1-1.2 μm region,” IEEE J. Sel. Top. Quantum Electron. 1(1), 2–13 (1995).
[Crossref]

K. Isobe, A. Suda, M. Tanaka, H. Hashimoto, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, “Nonlinear optical microscopy and spectroscopy employing octave spanning pulses,” IEEE J. Sel. Top. Quantum Electron. 16(4), 767–780 (2010).
[Crossref]

IEEE Photonics Technol. Lett. (1)

E. Desurvire, “Analysis of gain difference between forward- and backward-pumped erbium-doped fiber amplifiers in the saturation regime,” IEEE Photonics Technol. Lett. 4(7), 711–714 (1992).
[Crossref]

J. Opt. Soc. Am. A (1)

J. Opt. Soc. Am. B (1)

J. Phys. Chem. (1)

J. Widengren, U. Mets, and R. Rigler, “Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study,” J. Phys. Chem. 99(36), 13368–13379 (1995).
[Crossref]

Jpn. J. Appl. Phys. (1)

K. Isobe, K. Toda, Q. Song, F. Kannari, H. Kawano, A. Miyawaki, and K. Midorikawa, “Temporal focusing microscopy combined with three-dimensional structured illumination,” Jpn. J. Appl. Phys. 56(5), 052501 (2017).
[Crossref]

Lasers Surg. Med. (1)

J.-P. Ritz, A. Roggan, C. Isbert, G. Müller, H. J. Buhr, and C.-T. Germer, “Optical properties of native and coagulated porcine liver tissue between 400 and 2400 nm,” Lasers Surg. Med. 29(3), 205–212 (2001).
[Crossref] [PubMed]

Nat. Methods (1)

G. Donnert, C. Eggeling, and S. W. Hell, “Major signal increase in fluorescence microscopy through dark-state relaxation,” Nat. Methods 4(1), 81–86 (2007).
[Crossref] [PubMed]

Nat. Photonics (1)

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structures within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref] [PubMed]

Nucleic Acids Res. (1)

J. Kapuściński and K. Yanagi, “Selective staining by 4’, 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels,” Nucleic Acids Res. 6(11), 3535–3542 (1979).
[Crossref] [PubMed]

Opt. Express (10)

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005).
[Crossref] [PubMed]

M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing for axial scanning,” Opt. Express 14(25), 12243–12254 (2006).
[Crossref] [PubMed]

G. Olivié, D. Giguère, F. Vidal, T. Ozaki, J.-C. Kieffer, O. Nada, and I. Brunette, “Wavelength dependence of femtosecond laser ablation threshold of corneal stroma,” Opt. Express 16(6), 4121–4129 (2008).
[Crossref] [PubMed]

X. Zhou, D. Yoshitomi, Y. Kobayashi, and K. Torizuka, “Generation of 28-fs pulses from a mode-locked ytterbium fiber oscillator,” Opt. Express 16(10), 7055–7059 (2008).
[Crossref] [PubMed]

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

D. Fittinghoff, P. Wiseman, and J. Squier, “Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy,” Opt. Express 7(8), 273–279 (2000).
[Crossref] [PubMed]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

J. Zhao, W. Li, C. Wang, Y. Liu, and H. Zeng, “Pre-chirping management of a self-similar Yb-fiber amplifier towards 80 W average power with sub-40 fs pulse generation,” Opt. Express 22(26), 32214–32219 (2014).
[Crossref] [PubMed]

Y. Chiba, H. Takada, K. Torizuka, and K. Misawa, “65-fs Yb-doped fiber laser system with gain-narrowing compensation,” Opt. Express 23(5), 6809–6814 (2015).
[Crossref] [PubMed]

Opt. Lett. (1)

Rev. Sci. Instrum. (1)

Q. Song, A. Nakamura, K. Hirosawa, K. Isobe, K. Midorikawa, and F. Kannari, “Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy,” Rev. Sci. Instrum. 86(8), 083701 (2015).
[Crossref] [PubMed]

Other (1)

A. Nagler, “Plossl type eyepiece for use in astronomical instruments,” US Patent 4,482,217 (1984).

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (7)

Fig. 1
Fig. 1 Schematic of Yb-fiber CPA system. LPF: longpass filter, PBS: polarizing beamsplitter, PMSCF: polarization-maintaining single-clad fiber, PM-DCF: polarization-maintaining double-clad fiber, DM: dichroic mirror, LD: laser diode, WDM: wavelength-division multiplexer, PD: photodiode, GLP: Glan laser polarizer, AOM: acousto-optic modulator. HWP: halfwave plate, PM-MPC: polarization-maintaining multimode power combiner, PM LMA PCF: polarization-maintaining large-mode-area photonic crystal fiber.
Fig. 2
Fig. 2 (a) Spectra of the input seed pulse (black) to the CPA system and the output pulse from the CPA system with (red) and without (blue) the LPFs. (b) Optimized phase mask to compensate for the higher-order dispersion. (c) Measured IAC trace (black) and the upper and lower envelopes of the IAC trace calculated by the FTL pulse. (d) Extracted AC trace from the measured IAC trace in c and Sech2 fitted AC trace.
Fig. 3
Fig. 3 Schematic of the TF microscope. DM: dichroic mirror, SPF: short-pass filter, BPF: bandpass filter.
Fig. 4
Fig. 4 Signal distribution of one layer of 200-nm fluorescent beads with (a) a linear scale and (b) a semi-logarithmic scale.
Fig. 5
Fig. 5 (a, b) x-z cross-sectional images of the interface between a cover slip and fluorescent dye solution measured by (a) 2PEF-TF and (b) 3PEF-TF microscopies. (c, d) Comparison of signal distribution along the axial (z) direction recorded using 2PEF-TF and 3PEF-TF microscopies plotted on (c) a linear scale and (d) a semi-logarithmic scale.
Fig. 6
Fig. 6 x-y cross-sectional images of 1-µm beads obtained using (a) 3PEF-TF, (b) 2PEF-TF microscopies, and (c) merged image of (a) and (b).
Fig. 7
Fig. 7 Dual-color images of fixed mouse brain tissue stained with DAPI and SYTO83. (a, b) x-y cross-sectional (a) 3PEF and (b) 2PEF images acquired at a depth of 30 µm. (c, d) x-z cross-sectional (c) 3PEF and (d) 2PEF images along the dotted lines indicated in (a, b).

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

R ( 2 ) ( z ) 1 τ ( z ) 1 1 + ( z z R ) 2 ,
R ( 3 ) ( z ) I 3 ( z , t ) d t I p e a k 3 ( z ) τ ( z ) 1 τ 2 ( z ) 1 1 + ( z z R ) 2 ,

Metrics