Several methodologies have been developed over the past several years for
super-resolution fluorescence microscopy including saturated structured-illumination
microscopy (SSIM), stimulated emission depletion microscopy (STED), photoactivated
localization microscopy (PALM), fluorescence photoactivation localization microscopy
(FPALM), and stochastic optical reconstruction microscopy (STORM). While they have
shown great promise for biological research, these techniques all have individual
strengths and weaknesses. This review will describe the basic principles for
achieving super resolution, demonstrate some applications in biology, and provide an
overview of technical considerations for implementing these methods.
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