Multiplicative scatter correction (MSC) is a widely used normalization technique. It aims to correct spectra in such a way that they are as close as possible to a reference spectrum, generally the mean of the data set, by changing the scale and the offset of the spectra. When there are other differences in the spectra than just a scale and an offset, the mean spectrum changes after MSC. As a result, another MSC, with the new mean spectrum as the reference, will result in an additional correction. This paper studies the effect of multiple applications of MSC.

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