The capability of FT-IR transmission spectrometry was examined for the direct determination of glucose in whole blood without any sample preparation. For these investigations, the whole blood samples were automatically aspirated by a syringe pump into the transmission cell. Infrared spectra were recorded in the 1500-900 cm-1 range. Despite the high water background absorption and the complex blood matrix, significant spectral changes due to different glucose concentrations were observed. Chemometric (partial leastsquares) models were applied for the determination of glucose. A standard error of calibration of 13.8 mg/dL was obtained by using a partial least-squares calibration model containing five ranks. The residues for an independent test set were less than 15 mg/dL.

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