Stigmata of Crocus sativus L. were extracted in water, and the extract was analyzed by using a high-pressure liquid chromatography (HPLC) method. The carotenoids of saffron [ cis and trans glycosyl esters of crocetin or crocins (CRCs)] were isolated, and the two major isomer compounds (all- trans -digentiobiosyl crocetin and 13- cis -digentiobiosyl crocetin) were characterized spectroscopically with ultraviolet-visible (UV-vis), Fourier transform (FT)-Raman, and 1H NMR spectroscopies. The 13- cis compound showed in a UVvis spectrum an additional peak at 330 nm, in an FT-Raman spectrum the presence of a peak at 1138 cm 1, and in the 1H NMR downfield shifts of protons 12 and 15 and an upfield shift of proton 14, with respect to the all- trans isomer, according to differences in their skeletal structures. These features are shown also by the profile of their two-dimensional spectra.
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
Login to access OSA Member Subscription