One of the most indefinite points in analytical procedures for the determination of metallic constituents in biological material has been the reproducible dimensioning of a sample to allow the calculation of percent content on a valid basis. The mere weighing of fresh or frozen tissue does not in our opinion, constitute adequate dimensioning of a biological sample. The pathological sampling is subject to the variable fluid content of tissues at the moment of death and the autopsy technique employed. Subsequent handling and/or storage of the sample effects the fluid content of the issues in an uncontrolled manner. In consideration of these points an attempt was made to process biological material in a manner resulting in a competently dimensioned sample.

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