Abstract

We describe the performance of a high-throughput system for detecting Raman spectra excited in the range from 320 to 750 nm. The system utilizes notch filters to reject Rayleigh scattering, a single polychromator as the dispersive element, and photon detection via a charge-coupled device. The filters, mirrors, and grating are changed to maximize performance in each excitation region. The excitation source consists of a Kr<sup>+</sup> ion laser. Good-quality Raman data are reported for rhodamine 6G at 0.1 mM in methanol with the use of 752.5-nm excitation, and 0.2 mM flavin adenine dinucleotide (FAD) in tris buffer with the use of 647.1-nm excitation. High-quality resonance Raman data for 0.1 mM N-acetylglycine ethyl dithio ester in 5% CH<sub>3</sub>CN/H<sub>2</sub>O are also reported with the use of 324.0-nm excitation.

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