Abstract

Many studies have performed imaging under partially coherent illumination. However, to the best of our knowledge, there are no imaging methods for complicated objects such as cell colonies, which are large and diffract light multiple times. In this paper, we propose an image calculation method for the partially coherent illumination of a large-scale three-dimensional multi-diffractive object. The image is calculated via the summation of coherent images of each illumination angle using the beam propagation method. We apply this method to various microscopic observations, including phase contrast and differential interference contrast. Our method shows excellent agreement with experimental images of a bead and a photonic crystal fiber. As with a cell colony, our method reproduced the characteristics of the image through experimentation. Finally, we discuss the accuracy and the restriction conditions of our method.

© 2019 Optical Society of America

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