Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Noninvasive assessment of skin structure by combined photothermal radiometry and optical spectroscopy: coregistration with multiphoton microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We are combining two optical techniques, pulsed photothermal radiometry (PPTR) and diffuse reflectance spectroscopy (DRS), for noninvasive assessment of the structure and composition of human skin in vivo. The analysis involves simultaneous multidimensional fitting of the measured PPTR signals and DRS spectra with predictions of a numerical model of light transport (Monte Carlo) in a four-layer model optical model of human skin, accounting for the epidermis, papillary and reticular dermis, and subcutis. The assessed epidermal thickness values were tested by coregistration with a multiphoton microscope, which provides vertical sectioning capability based on two-photon excited fluorescence and second-harmonic generation in selected skin components. The comparison shows that these values correspond well to the maximal epidermal thicknesses measured in the multiphoton microscopy images, the rete ridges.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Physiological and structural characterization of human skin in vivo using combined photothermal radiometry and diffuse reflectance spectroscopy

Nina Verdel, Ana Marin, Matija Milanič, and Boris Majaron
Biomed. Opt. Express 10(2) 944-960 (2019)

Predictive model for the quantitative analysis of human skin using photothermal radiometry and diffuse reflectance spectroscopy

Nina Verdel, Jovan Tanevski, Sašo Džeroski, and Boris Majaron
Biomed. Opt. Express 11(3) 1679-1696 (2020)

Depth determination of chromophores in human skin by pulsed photothermal radiometry

Thomas E. Milner, Derek J. Smithies, Dennis M. Goodman, Alice Lau, and J. Stuart Nelson
Appl. Opt. 35(19) 3379-3385 (1996)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved