Out-of-Phase Imaging after Optical Modulation for Micro-and Macro-scale Multiplexed Fluorescence Imaging Against Autofluorescence and Ambient Light

Ruikang Zhang; Raja Chouket; Jérome Quérard; Marie-Aude Plamont; Zsolt Kelemen; Agathe Espagne; Alison G. Tebo; Arnaud Gautier; Lionel Gissot; Jean-Denis Faure; Vincent Croquette; Thomas Le Saux; Ludovic Jullien

doi:10.1364/NTM.2019.NS2B.4

Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)
OSA Technical Digest (Optica Publishing Group, 2019),
paper NS2B.4
•https://doi.org/10.1364/NTM.2019.NS2B.4

Out-of-Phase Imaging after Optical Modulation for Micro-and Macro-scale Multiplexed Fluorescence Imaging Against Autofluorescence and Ambient Light

Ruikang Zhang, Raja Chouket, Jérome Quérard, Marie-Aude Plamont, Zsolt Kelemen, Agathe Espagne, Alison G. Tebo, Arnaud Gautier, Lionel Gissot, Jean-Denis Faure, Vincent Croquette, Thomas Le Saux, and Ludovic Jullien

Novel Techniques in Microscopy 2019

Tucson, Arizona United States
14–17 April 2019
ISBN: 978-1-943580-54-5

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New Technologies (NS2B)

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R. Zhang, R. Chouket, J. Quérard, M. Plamont, Z. Kelemen, A. Espagne, A. G. Tebo, A. Gautier, L. Gissot, J. Faure, V. Croquette, T. Le Saux, and L. Jullien, "Out-of-Phase Imaging after Optical Modulation for Micro-and Macro-scale Multiplexed Fluorescence Imaging Against Autofluorescence and Ambient Light," in Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP), OSA Technical Digest (Optica Publishing Group, 2019), paper NS2B.4.

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Abstract

In micro-and macro-scale fluorescence imaging, Speed Out-of-Phase Imaging after Optical Modulation (Speed OPIOM) is shown to be highly efficient for multiplexed fluorescence imaging in the presence of autofluorescence and ambient light.

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