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FRET Efficiency Measurement with a Low-Cost Frequency Domain Fluorescence Lifetime Imaging Microscope

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Abstract

We utilize a frequency-domain FLIM system operating at a single modulation frequency in combination with a FRET standards calibration curve to measure the FRET efficiency of the vinculin tension sensor, VinTS, at the focal adhesions of living cells.

© 2020 The Author(s)

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