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Solving the Refractive Index – Thickness Ambiguity in Quantitative Phase Imaging of Primary Neurons in Culture with a Low-Cost Custom-Made 3D-Printed Perfusion Chamber

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Abstract

We developed a low-cost custom-made 3D-printed perfusion chamber to solve the refractive index – thickness ambiguity for quantitative phase imaging of primary neurons in culture. We also show successful decoupling experiments during hypo-osmotic and hyper-osmotic challenges.

© 2018 The Author(s)

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