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  • Imaging and Applied Optics 2018 (3D, AO, AIO, COSI, DH, IS, LACSEA, LS&C, MATH, pcAOP)
  • OSA Technical Digest (Optica Publishing Group, 2018),
  • paper JTu5B.5
  • https://doi.org/10.1364/3D.2018.JTu5B.5

Two-Photon light-sheet microscope with adaptive optics in the illumination and detection path

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Abstract

A light-sheet fluorescence microscope is presented that uses adaptive optics to enable diffraction limited imaging in Zebrafish embryos and tumor spheroids. Using two-photon excitation, a robust and sensorless wavefront optimization scheme is implemented.

© 2018 The Author(s)

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