We present results of experimental analysis of major fluorescence characteristics, namely spectral band, intensity and lifetime(s), of the two alkyl derivatives of fluorescein, MitoFluo and C8-FL. The fluorescence was monitored both in the pure buffer solution and with addition of the two types of liposomes, DOPC and soybeanPC+20%Cardiolipin. DOPC liposomes were shown to cause a shift of the fluorescence maximum and a change of the characteristic lifetime along with the appearance of an additional fluorescence component with a shorter lifetime for both fluorescein derivatives, while soy- beanPC+20%Cardiolipin liposomes provided neither of these effects.

© 2018 The Author(s)

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