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Conferences > FiO > 2010 > FML > FML2

Image Mapping Spectrometer (IMS) for Real Time Hyperspectral Fluorescence Microscopy

Liang Gao, Amicia D. Elliott, Robert T. Kester, Noah Bedard, Nathan Hagen, David W. Piston, and Tomasz S. Tkaczyk

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Abstract

Image Mapping Spectrometer (IMS) is a snapshot hyperspectral imaging technique capable of providing full spectral information for entire fluorescent sample within a short 100-200 ms integration time. This paper presents an IMS system acquiring, analyzing and displaying datacube at 5-10 frame/sec rates. System design and imaging results for cells expressing GFP/YFP/CFP are discussed.

© 2010 Optical Society of America

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