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Improving 2-Photon Microscopy by Beam Multiplexing and extended Excitation Bandwidth

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Abstract

We report technical improvements that increase frame rate, excitation bandwidth and penetration depth of 2-photon microscopes significantly. First, we implemented a flat optics beam splitter into the beam path for multi beam scanning to increase the frame rate by more than an order of magnitude. Second, we integrated an Optical Parametric Oscillator into the 2-photon microscope to extend the excitation bandwidth to longer wavelengths. Therefore penetration depth increases, red dyes/proteins can be excited and THG scattering delivers substantial structural information without staining.

© 2010 Optical Society of America

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