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Determination of optical properties of a two-layered epithelial model in UV-VIS

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Abstract

A combination of a variable -angle illumination and collection geometry and a Monte Carlo based light transport model was used to extract optical properties from a two-layered epithelial tissue model in the UV-Visible spectrum. This approach was validated using independent Monte Carlo simulations. The results indicated that the absorption coefficients were estimated with < 10% error and the scattering coefficients were estimated with < 15% error for both layers. The top layer thickness can be estimated with < 20% error for the thickness range from 200 µm to 500 µm.

© 2005 Optical Society of America

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