Imaging depth limit analysis in multiphoton microscopy using the beam propagation method

Xiaojun Cheng; Xiaojun Cheng; Sanaz Sadegh; Sharvari Zilpelwar; Sharvari Zilpelwar; Anna Devor; Anna Devor; Anna Devor; Lei Tian; Lei Tian; David A. Boas; David A. Boas

doi:10.1364/BRAIN.2020.BW2C.4

Biophotonics Congress: Biomedical Optics 2020 (Translational, Microscopy, OCT, OTS, BRAIN)
OSA Technical Digest (Optica Publishing Group, 2020),
paper BW2C.4
•https://doi.org/10.1364/BRAIN.2020.BW2C.4

Imaging depth limit analysis in multiphoton microscopy using the beam propagation method

Xiaojun Cheng, Sanaz Sadegh, Sharvari Zilpelwar, Anna Devor, Lei Tian, and David A. Boas

Optics and the Brain 2020

Washington, DC United States
20–23 April 2020
ISBN: 978-1-943580-74-3

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Scattering: Windows, Models, and More (BW2C)

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X. Cheng, S. Sadegh, S. Zilpelwar, A. Devor, L. Tian, and D. A. Boas, "Imaging depth limit analysis in multiphoton microscopy using the beam propagation method," in Biophotonics Congress: Biomedical Optics 2020 (Translational, Microscopy, OCT, OTS, BRAIN), OSA Technical Digest (Optica Publishing Group, 2020), paper BW2C.4.

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Abstract

We apply our recently developed beam propagation model to simulate wave propagation in scattering biological tissue. The imaging depth limit is estimated for two-photon, three-photon, and non-degenerate two-photon microscopy.

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